Research solutions for COVID-19

HTG molecular Diagnostics is committed to advancing the scientific understanding of the impact of the coronavirus SARS-CoV-2 and subsequent disease on human health worldwide. As a leader in Next Generation Sequencing (NGS) based gene expression profiling (GEP), we want to support our customers as they shift their work to address urgent needs around therapeutic and vaccine development through investigation of the body’s response to viral pathogenesis.

HTG offers multiple panels to support research investigating gene expression in response to SARS-CoV-2 infection, covering both mRNA and miRNA profiling.

HTG Panels for Sars-Cov-2

mRNA Profiling with the HTG AutoImmune Panel

The panel is a targeted gene expression profiling tool that contains 2,002 probes designed to identify genes involved in the body’s immune response to disease. This panel is equally suited to evaluate gene expression in response to autoimmune disease as it is to bacterial and viral infection, including infection from SARS-CoV-2 and the resulting COVID-19 disease.

  • Evaluate pathways, processes, and cell types involved in disease, including immune system and cytokine dysregulation in response to pathogenic infection.
  • Identify candidate targets involved in transcription, metabolism and viral defense
  • Discover and validate disease- and therapeutic- specific biomarkers
  • Measure responses to drug and immune therapy
  • Elucidate therapeutic mechanism of action or validate drug targets
  • Develop predictive signatures of infection and drug response
  • Moure coverage than naostrings

Sample Type

Input amount per well


500 µL


6.0 mm2

Extracted RNA

35 ng

Note: For nasopharyngeal swabs, RNA can be extracted fusing a standard RNA extraction kit or swab samples can be utilized following the HTG EdgeSeq extraction-free sample preparation.

Probes to genes involved in: Angiogenesis, endothelial activation, apoptosis, B-cell receptor signaling, cell cycle and proliferation, cell type markers, chemokine signaling, cytokines and cytokine dysregulation, cytotoxic receptors and function, DNA damage sensing and repair, epigenetics and transcriptional regulation, Fc receptors and phagocytosis, general immune response, immunometabolism, inflammasomes, lymphocyte trafficking, MCH Class I and Class II antigen presentation, mTOr signaling, NF-kB signaling, NOD-like receptor signaling, general interleukin signaling, oxidative stress, T-cell checkpoint modulation, T-cell receptor signaling, TGF-beta signaling, TH1/ TH2 / TH17 polarization and response, TNF signaling, Toll-like receptor signaling, Treg differentiation, Type I / Type II interferon signaling, vesicle and endocytosis.

microRNA Profiling with the    microRNA Panel

Enables researchers to measure the expression of 2,083 human miRNA transcripts using next-generation sequencing (NGS). The HTG EdgeSeq miRNA WTA provides excellent coverage of miRNA transcripts with more than 80% of all mature transcripts identified and almost 90% of the high confidence miRNA transcripts identified.

  • Determine detailed analysis of virus-host interactions to understand viral pathogenesis and determine outcomes of infection
  • Discover and validate diagnostic- and therapeutic- specific biomarkers
  • Measure miRNA responses to therapies
  • Identify changes in epigenetic regulation caused by viral infection
  • Understand miRNA mode of actions and their roles during viral infections 
  • Identify options for the treatment of COVID-19
  • Develop predictive signatures of infection and drug response
  • Determine miRNA-mediated interaction between the hosts and virus

Sample Type

Input amount per well

FFPE Tissue

One 5 µm section

Extracted RNA

35 ng


500 µl

Plasma / Serum

≥15 µl


≥3,000 cells

Adaptive immune response, angiogenesis, apoptosis, calcium ion transport, cell cycle, cell migration, cell proliferation, cellular response to hypoxia, cellular senescence, chemokine production and secretion endocytosis, gene silencing, hypoxia, IFN anti-viral activity, immune cell mediated cytotoxicity, immune cell proliferation, immune response, inhibition of translation, interferon-gamma production and secretion, interleukin production and secretion, JAK/STAT signaling, MAPK signaling, proliferation, regulation of cellular differentiation, response to virus, vascularization.

Analyze your COVID-19 response data

The HTG EdgeSeq Reveal is a powerful, simple-to-use software platform for interrogating and visualizing HTG EdgeSeq NGS data. It is purpose built to help researchers Gain insights into complex biology and gene signatures that enable a greater understanding of disease heterogeneity and host / disease state interactions.

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Quality control metrics

Assesses several key assay performance metrics to determine the quality of the data produced or detect technical errors in an HTG EdgeSeq run

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Principle components analysis

PCA is widely used to emphasize variation and capture strong patterns in a data set; it is an exploratory tool used to analyze data; make predictive models and to visualize relatedness between populations.

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Differential expression

The box plot allows quick graphical examination of one or more data sets; particularly useful for comparing distributions between several groups or sets of data. If higher variation is visible in one group this will diminish statistical significance but may indicate an important biological process
(differential variability).

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Volcano plots

 A volcano plot is used to quickly identify meaningful changes between two conditions in large data sets.

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Heat maps

 A heat map is used to visualize relative expression of many genes across comparable or related samples.


Normalization and principal component analysis tools: